Transcriptome and Proteome Analysis Identifies Salt Stress Response Genes in Bottle Gourd Rootstock-Grafted Watermelon Seedlings
نویسندگان
چکیده
Soil salinization poses a huge challenge to the development of agriculture and seriously decreases crop yield quality. In recent years, grafting has become one key agronomic techniques used enhance plant abiotic stress tolerance. this study, we found that watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] grafted onto bottle gourd (Lagenaria siceraria Standl.) significantly enhanced salt Transcriptome analysis revealed total 8462 differentially expressed genes (DEGs) were identified, number up- down-regulated 3207 5255, respectively. The DEGs in rootstock-grafted plants mainly involved carbon metabolism, photosynthesis, hormone signal transduction. Furthermore, proteome identified 28 differently proteins (DEPs) under stress. These DEPs closely associated with amino acid protein synthesis, mitochondrial metabolism defense. Combined transcriptome analyses showed stress-responded seedlings transduction, synthesis pathways.
منابع مشابه
Proteomic Study Related to Vascular Connections in Watermelon Scions Grafted onto Bottle-Gourd Rootstock under Different Light Intensities
Although grafting is broadly used in the production of crops, no information is available about the proteins involved in vascular connections between rootstock and scion. Similarly, proteome changes under the light intensities widely used for grafted seedlings are of practical use. The objective of this study was to determine the proteome of vascular connections using watermelon (Citrullus vulg...
متن کاملIdentification of Appropriate Reference Genes for Normalization of miRNA Expression in Grafted Watermelon Plants under Different Nutrient Stresses
Watermelon (Citrullus lanatus) is a globally important crop belonging to the family Cucurbitaceae. The grafting technique is commonly used to improve its tolerance to stress, as well as to enhance its nutrient uptake and utilization. It is believed that miRNA is most likely involved in its nutrient-starvation response as a graft-transportable signal. The quantitative real-time reverse transcrip...
متن کاملRoot Exudates from Grafted-Root Watermelon Showed a Certain Contribution in Inhibiting Fusarium oxysporum f. sp. niveum
Grafting watermelon onto bottle gourd rootstock is commonly used method to generate resistance to Fusarium oxysporum f. sp. niveum (FON), but knowledge of the effect of the root exudates of grafted watermelon on this soil-borne pathogen in rhizosphere remains limited. To investigate the root exudate profiles of the own-root bottle gourd, grafted-root watermelon and own-root watermelon, recircul...
متن کاملResponse of Almond Genotypes/Cultivars Grafted on GN15 ‘Garnem’ Rootstock in Deficit-Irrigation Stress Conditions
This study was conducted to evaluate the response of Iranian promisinglate blooming almond genotypes to deficit-irrigation stress on GN15 rootstock. One-year old plants subjectedto three deficit-irrigation, including moderate and severe stress (soil water potential, Ψsoil = -0.8 and -1.6 MPa, respectively) and a control treatment (Ψsoil= -0.33 MPa), were applied for six weeks to five grafting c...
متن کاملSodium Distribution in Salt-stressed Citrus Rootstock Seedlings
Although citrus trees are considered relatively salt-sensitive, there are consistent differences in Na and Cl tolerance among different citrus rootstocks. We grew uniform seedlings of rough lemon (RL) and the more Na-tolerant Swingle citrumelo (SC) with and without 50 mM NaCl for 42 days. Salinity reduced leaf chlorophyll and plant transpiration rate (Ep) more in RL than SC. Confocal laser scan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agronomy
سال: 2023
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy13030618